The CD31 antibody is glycoprotein expressed in platelets and endothelial cells. It may also be involved in cell adhesion and cell signaling. It is designed to be used for research and not in diagnostic procedures and has no known clones. It is a Rabbit IgG isotype with an undetermined epitope. Likewise, it is a synthetic peptide that corresponds to the C-terminus of the mouse protein by the same name. It has a molecular weight of 130 and 100 kDa and has been tested in humans.
The Application Methods
It is designed to be used with Immunohistochemistry applications. The positive control is the tonsil, and the cellular localization is in the membrane. It is best to follow your protocols designed by your laboratory. However, it is preferable to prepare the specimen using paraffin embedded, or Formalin fixed tissue. Slides should be deparaffinized using xylene and/or graded alcohols. You can find a variety of formula options, but when using the concentrated version, you should dilute the CD31 antibody using a ratio of 1:50, though this is an estimate.
To retrieve the antigen, you’ll need to boil the section of tissue using a 10mM citrate buffer with a pH of 6.0 for at least 10 minutes, allowing it to cool to room temperature for 20 minutes.
Likewise, the antibody will need to be in incubation for at least 30 minutes at room temperature. It is best to wash the slides between steps using a mild detergent and then rinse them with PBS-Tween, diluted appropriately. Use your visualization system to detect the CD31 antibody.
Many laboratories find it easier to use the pre-diluted formula because they don’t have to use another step. Because it is approved for one application (IHC) only, you can purchase the pre-diluted version. Otherwise, you can buy 0.1, 0.5, or 1.0 ml of the antibody and dilute it yourself as needed.
The CD31 antibody can be found at Spring Bioscience. Visit now to learn more.
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